Large DNA Template Dependent Error Variation During Transcription
2014 (English)In: Biophysics and Structure to Counter Threats and Challenges / [ed] Joseph D. Puglisi, Manolia V. Margaris, Springer Netherlands, 2014, , 19 p.39-57 p.Chapter in book (Other academic)
The accuracy of an enzymatic reaction system is the propensity toprocess the correct, or cognate, substrate over similar, non-cognate substrates. Thisis of particular importance to polymerization reactions with a template sequence,like transcription, translation and replication. A theoretical framework for theanalysis of accuracy control is presented, including initial substrate selection andkinetic proofreading. This framework allows for analysis not only of the efficiencyof accuracy control, but also its source in standard free energy differences andequilibrium constants and its relation to the rate of product formation. A key featureis the separation of the selection in a context dependent discard parameter anda context independent discrimination parameter. When the theory is applied tothe example of prokaryote transcription, it is shown that the discard parameter,composed by experimentally well-defined values, induces a large template sequencedependent error rate variation.
Place, publisher, year, edition, pages
Springer Netherlands, 2014. , 19 p.39-57 p.
, NATO Science for Peace and Security Series B: Physics and Biophysics, ISSN 1874-6500
Biochemistry and Molecular Biology
Research subject Biology with specialization in Molecular Biotechnology
IdentifiersURN: urn:nbn:se:uu:diva-181002DOI: 10.1007/978-94-007-4923-8_3ISBN: 978-94-007-4922-1OAI: oai:DiVA.org:uu-181002DiVA: diva2:552627
Biophysics and Structure to Counter Threats and Challenges