Optimum drift velocity for single molecule fluorescence bursts in micro/nano-fluidic channels
2012 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 101, no 4, 043120- p.Article in journal (Refereed) Published
Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This paper is a sequel to an earlier one of ours [L. L. Kish et al., Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity that minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications.
Place, publisher, year, edition, pages
2012. Vol. 101, no 4, 043120- p.
Physical Sciences Engineering and Technology
Research subject Engineering Science with specialization in Solid State Physics
IdentifiersURN: urn:nbn:se:uu:diva-181939DOI: 10.1063/1.4739471ISI: 000306944700097OAI: oai:DiVA.org:uu-181939DiVA: diva2:558234