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Optimum drift velocity for single molecule fluorescence bursts in micro/nano-fluidic channels
Dept of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
Dept of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Dept of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
2012 (English)In: Applied Physics Letters, ISSN 0003-6951, E-ISSN 1077-3118, Vol. 101, no 4, 043120- p.Article in journal (Refereed) Published
Abstract [en]

Photonic burst histograms can be used to identify single protein molecules in micro/nano-fluidic channels provided the width of the histogram is narrow. Photonic shot noise and residence time fluctuations, caused by longitudinal diffusion, are the major sources of the histogram width. This paper is a sequel to an earlier one of ours [L. L. Kish et al., Appl. Phys. Lett. 99, 143121 (2011)] and demonstrates that, for a given diffusion coefficient, an increase of the drift velocity enhances the relative shot noise and decreases the relative residence time fluctuations. This leads to an optimum drift velocity that minimizes the histogram width and maximizes the ability to identify single molecules, which is an important result for applications.

Place, publisher, year, edition, pages
2012. Vol. 101, no 4, 043120- p.
National Category
Physical Sciences Engineering and Technology
Research subject
Engineering Science with specialization in Solid State Physics
Identifiers
URN: urn:nbn:se:uu:diva-181939DOI: 10.1063/1.4739471ISI: 000306944700097OAI: oai:DiVA.org:uu-181939DiVA: diva2:558234
Available from: 2012-10-02 Created: 2012-10-02 Last updated: 2017-12-07Bibliographically approved

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Granqvist, Claes G.

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