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The influence of collagen density in cellular distribution
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - Ångström, Polymer Chemistry.
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2012 (English)In: Journal of Tissue Engineering and Regenerative Medicine, ISSN 1932-6254, Vol. 6, no suppl 1, 166-166 p.Article in journal, Meeting abstract (Other academic) Published
Abstract [en]

Adequate cellular in-growth into biomaterials is one of the fundamental requirements in regenerative medicine. Type-I-collagen is the most commonly used material for soft tissue engineering, because it is nonimmunogenic and a highly porous network for cellular support. However, adequate cell in-growth and cell seeding has been suboptimal. Different densities of collagen scaffolds (0.3% to 0.8% (w/v)) with/without polymer knitting (poly-caprolactone (PCL)) were prepared. The structure of collagen scaffolds was characterized using scanning electronic microscopy (SEM) and HE staining. The mechanical strength of hybrid scaffolds was determined using tensile strength analysis. Cellular penetration and interconnectivity were evaluated using fluorescent bead distribution and human bladder smooth muscle cells and urothelium seeding. SEM and HE analysis showed the honeycomb structure and the hybrid scaffolds were adequately connected. The hybrid scaffolds were much stronger than collagen alone. The distribution of the beads and cells were highly dependent on the collagen density: at lower densities the beads and cells were more evenly distributed and penetrated deeper into the scaffold. The lower density collagen scaffolds showed remarkably deeper cellular penetration and by combining it with PCL knitting the tensile strength was enhanced. This study indicated that a 0.4% hybrid scaffold strengthened with knitting achieved the best cellular distribution.

Place, publisher, year, edition, pages
2012. Vol. 6, no suppl 1, 166-166 p.
National Category
Medical and Health Sciences Polymer Chemistry
Research subject
Chemistry with specialization in Polymer Chemistry
URN: urn:nbn:se:uu:diva-182408DOI: 10.1002/term.1586ISI: 000308313001213OAI: oai:DiVA.org:uu-182408DiVA: diva2:560283
Available from: 2012-10-12 Created: 2012-10-10 Last updated: 2013-01-08Bibliographically approved

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Hilborn, Jöns
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Polymer Chemistry
Medical and Health SciencesPolymer Chemistry

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