uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Effects of propofol on GABAA channel conductance in rat-cultured hippocampal neurons.
John Curtin School of Medical Research, Australian National University.
2003 (English)In: European Journal of Pharmacology, ISSN 0014-2999, E-ISSN 1879-0712, Vol. 468, no 2, 75-82 p.Article in journal (Refereed) Published
Abstract [en]

Channels were activated, in ripped-off patches from rat-cultured hippocampal neurons, by propofol alone, propofol plus 0.5 microM GABA (gamma-aminobutyric acid) or GABA alone. The propofol-activated currents were chloride-selective, showed outward-rectification and were enhanced by 1 microM diazepam. The maximum propofol-activated channel conductance increased with propofol concentration from less than 15 pS (10 microM) to about 60 pS (500 microM) but decreased to 40 pS in 1 mM propofol. Fitting the data from 10 to 500 microM propofol with a Hill-type equation gave a maximum conductance of 64 pS, an EC50 value of 32 microM and a Hill coefficient of 1.1. Addition of 0.5 microM GABA shifted the propofol EC50 value to 10 microM and increased the maximum channel conductance to about 100 pS. The Hill coefficient was 0.8. The maximum channel conductance did not increase further when 1 microM diazepam was added together with a saturating propofol concentration and GABA. The results are compared to effects other drugs have on GABAA channels conductance.

Place, publisher, year, edition, pages
2003. Vol. 468, no 2, 75-82 p.
National Category
Neurosciences Physiology
URN: urn:nbn:se:uu:diva-182719PubMedID: 12742514OAI: oai:DiVA.org:uu-182719DiVA: diva2:560648
Available from: 2012-10-15 Created: 2012-10-15 Last updated: 2012-10-15

Open Access in DiVA

No full text


Search in DiVA

By author/editor
Birnir, Bryndis
In the same journal
European Journal of Pharmacology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 277 hits
ReferencesLink to record
Permanent link

Direct link