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Keratinocyte-based cell assays: their potential pitfalls
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Dermatology and Venereology.
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2012 (English)In: Archives of Dermatological Research, ISSN 0340-3696, E-ISSN 1432-069X, Vol. 304, no 9, 765-768 p.Article in journal (Refereed) Published
Abstract [en]

As an in vitro model system, patient-derived epidermolysis bullosa simplex keratinocytes have had an immense impact on what we know today about keratin filament function and their role in disease development. In the absence of gene therapy, screening compound libraries for new or better drugs is another approach to improve existing treatments for genodermatoses. However in this study, we report of the potential pitfalls when using this type of cell lines as a "reporter" system. When cell lines with different genetic backgrounds are being used in cell-based assays, the greatest obstacle is to determine the most appropriate culture conditions (i.e., the composition of medium, number of cells plated and number of days in culture). We demonstrate how culture conditions can greatly interfere with the cellular response in cell-based assays (cell proliferation, metabolic activity and migration), potentially also giving rise to misleading data.

Place, publisher, year, edition, pages
2012. Vol. 304, no 9, 765-768 p.
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Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-183728DOI: 10.1007/s00403-012-1285-6ISI: 000310317800011PubMedID: 22983161OAI: oai:DiVA.org:uu-183728DiVA: diva2:563955
Available from: 2012-11-01 Created: 2012-11-01 Last updated: 2017-12-07Bibliographically approved

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Törmä, Hans

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