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Four chromosome replication origins in the archaeon Pyrobaculum calidifontis
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Molecular Evolution.
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2012 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 85, no 5, 986-995 p.Article in journal (Refereed) Published
Abstract [en]

Replication origins were mapped in hyperthermophilic crenarchaea, using high-throughput sequencing-based marker frequency analysis. We confirm previous origin mapping in Sulfolobus acidocaldarius, and demonstrate that the single chromosome of Pyrobaculum calidifontis contains four replication origins, the highest number detected in a prokaryotic organism. The relative positions of the origins in both organisms coincided with regions enriched in highly conserved (core) archaeal genes. We show that core gene distribution provides a useful tool for origin identification in archaea, and predict multiple replication origins in a range of species. One of the P. calidifontis origins was mapped in detail, and electrophoretic mobility shift assays demonstrated binding of the Cdc6/Orc1 replication initiator protein to a repeated sequence element, denoted Orb-1, within the origin. The high-throughput sequencing approach also allowed for an annotation update of both genomes, resulting in the restoration of open reading frames encoding proteins involved in, e.g., sugar, nitrate and energy metabolism, as well as in glycosylation and DNA repair.

Place, publisher, year, edition, pages
2012. Vol. 85, no 5, 986-995 p.
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Biological Sciences
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URN: urn:nbn:se:uu:diva-183785DOI: 10.1111/j.1365-2958.2012.08155.xISI: 000308089900013OAI: oai:DiVA.org:uu-183785DiVA: diva2:564133
Available from: 2012-11-01 Created: 2012-11-01 Last updated: 2017-12-07Bibliographically approved

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Pelve, Erik A.Lindås, Ann-ChristinKnöppel, AnnaBernander, Rolf

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