Invertebrate hematopoiesis: an anterior proliferation centre as a link between the hematopoietic tissue and the brain
2012 (English)In: Stem Cells and Development, ISSN 1547-3287, E-ISSN 1557-8534, Vol. 21, no 17, 3173-3186 p.Article in journal (Refereed) Published
During evolution, the innate and adaptive immune systems developed to protect organisms from nonself substances. The innate immune system is phylogenetically more ancient and is present in most multicellular organisms, whereas adaptive responses are restricted to vertebrates. Arthropods, lack the blood cells of the lymphoid lineage, and oxygen-carrying erythrocytes, making them suitable model animals to study the regulation of the blood cells of the innate immune system. Many crustaceans have a long life span and need to continuously synthesize blood cells, in contrast to many insects. The hematopoietic tissue (HPT) of Pacifastacus leniusculus provides a simple model to study hematopoiesis because the tissue can be isolated and the proliferation of stem cells and their differentiation can be studied both in vivo and in vitro. Here we demonstrate new findings of a physical link between the HPT and the brain. Actively proliferating cells were localized to an anterior proliferation centre (APC) in the anterior part of the tissue near the area linking the HPT to the brain, whereas more differentiated cells were detected in the posterior part. The central areas of HPT expand in response to lipopolysaccharide-induced blood loss. Cells isolated from the APC divide rapidly and form cell clusters in vitro; conversely, the cells from the remaining HPT form monolayers, and they can be induced to differentiate in vitro. Our findings offer an opportunity to learn more about invertebrate hematopoiesis and its connection to the central nervous system and thereby to obtain new information about the evolution of different blood and nerve cell lineages.
Place, publisher, year, edition, pages
2012. Vol. 21, no 17, 3173-3186 p.
Research subject Biology
IdentifiersURN: urn:nbn:se:uu:diva-174038DOI: 10.1089/scd.2012.0077ISI: 000310840500011PubMedID: 22564088OAI: oai:DiVA.org:uu-174038DiVA: diva2:565204
FunderSwedish Research Council, 621-2011-4797; 621-2009-5715; 319-2010-6250Formas, 223.2011-606