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A Role for the 2 ' OH of Peptidyl-tRNA Substrate in Peptide Release on the Ribosome Revealed through RF-Mediated Rescue
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Computational and Systems Biology.
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2012 (English)In: Chemistry and Biology, ISSN 1074-5521, E-ISSN 1879-1301, Vol. 19, no 8, 983-993 p.Article in journal (Refereed) Published
Abstract [en]

The 2' OH of the peptidyl-tRNA substrate is thought to be important for catalysis of both peptide bond formation and peptide release in the ribosomal active site. The release reaction also specifically depends on a release factor protein (RF) to hydrolyze the ester linkage of the peptidyl-tRNA upon recognition of stop codons in the A site. Here, we demonstrate that certain amino acid substitutions (in particular those containing hydroxyl or thiol groups) in the conserved GGQ glutamine of release factor RF1 can rescue defects in the release reaction associated with peptidyl-tRNA substrates lacking a 2' OH. We explored this rescue effect through biochemical and computational approaches that support a model where the 2' OH of the P-site substrate is critical for orienting the nucleophile in a hydrogen-bonding network productive for catalysis.

Place, publisher, year, edition, pages
2012. Vol. 19, no 8, 983-993 p.
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Biological Sciences
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URN: urn:nbn:se:uu:diva-183895DOI: 10.1016/j.chembiol.2012.06.011ISI: 000309152500010OAI: oai:DiVA.org:uu-183895DiVA: diva2:565404
Available from: 2012-11-07 Created: 2012-11-05 Last updated: 2017-12-07Bibliographically approved

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Trobro, StefanÅqvist, Johan

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