PCR-RFLP analysis of cpDNA in the genus Abies
1999 (English)In: Theor Appl Genet, Vol. 98, 802-808 p.Article in journal (Refereed) Published
We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome. Ten different chloroplast DNA regions, consisting of coding and non-coding DNA sequences, were amplified with specific primers in ten different Abies taxa. The amplification products were digested with several restriction enzymes. The results showed that the chloroplast genome is highly variable in mast of the investigated taxa and contains multiple variable regions that appear to be distributed throughout the whole genome. Species-diagnostic markers were found for four of the ten investigated species. Unexpectedly, intra-specific variation was also detected in four species. It is likely that further studies, including larger sample sizes and/or more powerful methods for the detection of chloroplast DNA variation. will reveal additional variation for this genus.
Place, publisher, year, edition, pages
1999. Vol. 98, 802-808 p.
Intra-Specific Variation, Genetic-Relationships, Pcr-Rflp Analysis, Phylogenetic Analysis, Pinus-Radiata, Inheritance, Chloroplast Dna, Mitochondrial, Haplotype, Cephalonica Loudon, Universal Primers, Chloroplast Dna Variation, Allozyme, Abies, Noncoding Regions
IdentifiersURN: urn:nbn:se:uu:diva-184544OAI: oai:DiVA.org:uu-184544DiVA: diva2:566592