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Visualization of custom-tailored iron oxide nanoparticles chemistry, uptake, and toxicity
Dept of Chemistry, SLU BioCenter, Uppsala.
FOI, Umeå.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Science for Life Laboratory, Cell Physics, Dept of Applied Physics, Albanova University center, KTH, Stockholm.
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2012 (English)In: Nanoscale, ISSN 2040-3364, Vol. 4, no 23, 7383-7393 p.Article in journal (Refereed) Published
Abstract [en]

Nanoparticles of iron oxide generated by wearing of vehicles have been modelled with a tailored solution of size-uniform engineered magnetite particles produced by the Bradley reaction, a solvothermal metal–organic approach rendering hydrophilic particles. The latter does not bear any pronounced surface charge in analogy with that originating from anthropogenic sources in the environment. Physicochemical properties of the nanoparticles were thoroughly characterized by a wide range of methods, including XPD, TEM, SEM, DLS and spectroscopic techniques. The magnetite nanoparticles were found to be sensitive for transformation into maghemite under ambient conditions. This process was clearly revealed by Raman spectroscopy for high surface energy magnetite particles containing minor impurities of the hydromaghemite phase and was followed by quantitative measurements with EXAFS spectroscopy. In order to assess the toxicological effects of the produced nanoparticles in humans, with and without surface modification with ATP (a model of bio-corona formed in alveolar liquid), a pathway of potential uptake and clearance was modelled with a sequence of in vitro studies using A549 lung epithelial cells, lymphocyte 221-B cells, and 293T embryonal kidney cells, respectively. Raman microscopy unambiguously showed that magnetite nanoparticles are internalized within the A549 cells after 24 h co-incubation, and that the ATP ligand is retained on the nanoparticles throughout the uptake process. The toxicity of the nanoparticles was estimated using confocal fluorescence microscopy and indicated no principal difference for unmodified and modified particles, but revealed considerably different biochemical responses. The IL-8 cytokine response was found to be significantly lower for the magnetite nanoparticles compared to TiO2, while an enhancement of ROS was observed, which was further increased for the ATP-modified nanoparticles, implicating involvement of the ATP signalling pathway in the epithelium.

Place, publisher, year, edition, pages
2012. Vol. 4, no 23, 7383-7393 p.
National Category
Nano Technology Inorganic Chemistry
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Solid State Physics
URN: urn:nbn:se:uu:diva-187493DOI: 10.1039/C2NR32572AISI: 000310978900015OAI: oai:DiVA.org:uu-187493DiVA: diva2:574882
Available from: 2012-12-06 Created: 2012-12-06 Last updated: 2013-01-28Bibliographically approved

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Ahlinder, LinneaButorin, Segei M.Österlund, Lars
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Solid State PhysicsDepartment of Physics and Astronomy
Nano TechnologyInorganic Chemistry

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