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Optimization of cyclotide extraction parameters
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Division of Pharmacognosy.
2012 (English)In: Phytochemistry Letters, ISSN 1874-3900, E-ISSN 1876-7486, Vol. 5, no 4, 776-781 p.Article in journal (Refereed) Published
Abstract [en]

Cyclotides are gene-encoded plant mini-proteins that contain a unique circular and cystine knotted amide backbone. Because of that ultra stable scaffold and the ability to harness a wide variety of sequences and biological activities within the scaffold, cyclotides find interesting potential applications for drug discovery and in agriculture. However, some fundamental knowledge is still missing to exploit these plant compounds, including finding the optimal process of their extraction from plant material. In the current work, the extraction parameters solvent type, time of extraction, number of re-macerations and the plant material to solvent ratio have been compared using the sweet violet (Viola odorata L.) as a model plant. That species is a well-characterized and rich source of cyclotides that contains prototypic cyclotides with different chemical and physical properties. We found that hydroalcoholic solutions of medium polarity give good yield of the cyclotide cocktail. In conclusion, single maceration with 50% MeOH for 6 h at a plant material to solvent ratio of 0.5:10 (g/mL) represents an optimum extraction method.

Place, publisher, year, edition, pages
2012. Vol. 5, no 4, 776-781 p.
Keyword [en]
Plant peptide, Cyclotide, Extraction, Optimization, Cystine knot, Solvent
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-189138DOI: 10.1016/j.phytol.2012.09.001ISI: 000311337300020OAI: oai:DiVA.org:uu-189138DiVA: diva2:582116
Available from: 2013-01-03 Created: 2012-12-25 Last updated: 2017-12-06Bibliographically approved

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Burman, RobertGöransson, Ulf

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