uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The crustacean cytokine astakine-1: a link between adult neurogenesis and hematopoiesis?
Wellesley Coll, Neurosci. Program, Wellesley.
Wellesley Coll, Neurosci. Program, Wellesley.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Comparative Physiology. (jämförande fysiologi)
Wellesley Coll, Neurosci. Program, Wellesley, MA 02181 USA .
2012 (English)Conference paper, Poster (Refereed)
Abstract [en]

Adult neurogenesis occurs in the brains of vertebrate and invertebrate species. In the crayfish Procambarus clarkii, neurogenic niches located on the ventral surface of the brain contain the 1st-generation precursors that produce adult-born neurons. These cells divide symmetrically and both daughter cells migrate in streams to proliferation zones in interneuronal cell clusters, where they divide at least one more time. Double-nucleoside labeling has confirmed that the 1st-generation neuronal precursors are not self-renewing. However, previous studies have shown that the niche is not depleted and, further, that niche cell numbers increase throughout the animal’s lifetime (Zhang et al., 2009). Our goal, therefore, is to identify the source of niche cells and in particular the 1st-generation neuronal precursors. Experiments have revealed that hemocytes, and in particular semi-granular cells, are attracted to and incorporated into the niches of dissected intact brains in culture; cell types from other tissues show no affinity for the niche (Benton et al., 2011). Lin et al. (2010) demonstrated that a prokineticin family cytokine, astakine-1, promotes the differentiation and release of semi-granular cells from hematopoietic tissue. In the present studies, we have injected astakine-1 into crayfish and examined subsequent changes in niche cell numbers, as well as BrdU incorporation into cells in the niche, streams and proliferation zones. By 48 hrs following astakine-1 injection, three alterations in the neurogenic system have been demonstrated: (1) increased total numbers of cells in the neurogenic niche compared to saline-injected shams; (2) increased BrdU-incorporation into cells in the niche, streams and proliferation zone in cell cluster 10; (3) increased probability of observing cells within the vascular cavity in the niche, implying that the numbers of cells interacting with the niche is upregulated. These experiments exploring the relationship between the hematopoietic system and adult neurogenesis suggest that cells released from hematopoietic tissues following astakine-1 injection influence the cellular composition of the niche and the rate of BrdU-incorporation into niche cells.

Place, publisher, year, edition, pages
2012. 427.13/A59- p.
National Category
Developmental Biology
URN: urn:nbn:se:uu:diva-189796OAI: oai:DiVA.org:uu-189796DiVA: diva2:582316
42nd Annual Meeting of the Society for Neuroscience. New Orleans, October 13 -17, 2012
Swedish Research Council, 621-2011-4797
Available from: 2013-01-04 Created: 2013-01-04 Last updated: 2015-07-30Bibliographically approved

Open Access in DiVA

No full text

Search in DiVA

By author/editor
Söderhäll, Irene
By organisation
Comparative Physiology
Developmental Biology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 300 hits
ReferencesLink to record
Permanent link

Direct link