Crustacean hematopoietic factor: a regulator of hemocyte homeostasis
2012 (English)Conference paper, Poster (Refereed)
Place, publisher, year, edition, pages
IdentifiersURN: urn:nbn:se:uu:diva-189813OAI: oai:DiVA.org:uu-189813DiVA: diva2:582337
The 12th Congress of the International Society of Developmental and Comparative Immunology was held at Hilton Fukuoka Sea Hawk Hotel, Fukuoka city, Japan, from 9th to 13th July, 2012.
Regulation of blood cell homeostasis of the innate immune system of crustaceans is regulated by the astakine cytokines. Astakines (astakine 1 and astakine 2) are among the first invertebrate cytokines shown to be involved in hematopoiesis, and they can stimulate the proliferation, differentiation, and survival of hematopoietic tissue cells. Studies of astakine-induced hematopoiesis in crustaceans have increased our understanding of the regulation of crustacean hematopoiesis as well as may also provide new evolutionary perspectives about this process. One important role of astakine 1 is to regulate the crossroad between proliferation/differentiation and apoptosis, and here we report the importance of a novel factor, named crustacean hematopoietic factor (CHF), in this process. This protein in was identified in the freshwater crayfish Pacifastacus leniusculus in a suppression subtractive hybridization after treatment of HPT cells with astakine 1. CHF is a 9 kDa cysteine-rich protein and share high similarity with the N-terminal region of vertebrate Cysteine-rich motor neuron 1 (CRIM1) and contains an unusual insulin growth factor binding protein motif of unknown function. Expression of CHF is induced in primary cell cultures of crayfish hematopoietic tissue (HPT) cells after treatment with astakine 1. Further, silencing of CHF induces increased apoptosis of cultured HPT cells. This factor is exclusively expressed in the hemocyte lineage of crayfish and in vivo RNA interference shows that knockdown of this gene results in severe loss of hemocytes and a high apoptotic rate in HPT. Thus crayfish CHF is critical for hemocyte survival and plays an important role in hemocyte homeostasis. Localization of CHF expression in the HPT further shows that the transcript is exclusively expressed in differentiating cells and it is also restricted to the semigranular cell lineage. This role of CHF may also lead to further studies to explore the function of the untypical insulin growth factor binding protein motif located in the N-terminal of vertebrate CRIM1.2013-01-042013-01-042013-01-07Bibliographically approved