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Reduced levels of E-cadherin correlate with progression of corticotroph pituitary tumours
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular and Morphological Pathology. (Department of Clinical Pathology and Cytology, Alafuzoff)
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2011 (English)In: Clinical Endocrinology, ISSN 0300-0664, E-ISSN 1365-2265, Vol. 75, no 6, 811-818 p.Article in journal (Refereed) Published
Abstract [en]

OBJECTIVES:

Loss of E-cadherin is an important marker of epithelial tumour progression. The aims of this study were to explore whether E-cadherin expression and localization correlate to corticotroph tumour progression, relate the expression of the E-cadherin gene (CDH1) to immunohistochemical E-cadherin staining pattern, and study whether the E-cadherin levels were correlated to methylation status of the CDH1 promoter region.

DESIGN:

Immunohistochemical analyses of E-cadherin protein were performed, as was RT-qPCR of the CDH1 and the POMC genes. Methylation pattern of the promoter region of CDH1 was measured using pyrosequencing of bisulfite-treated DNA.

PATIENTS:

Forty-five patients operated at a tertiary referral centre in Oslo, Norway. Adenoma tissue sections and RNA samples from patients with verified Cushing's disease or Nelson's syndrome were collected.

MEASUREMENTS:

Expression of E-cadherin mRNA and protein in pituitary corticotroph adenomas and average percentage of methylated cytosines in a cytosine-phosphate-guanosine island of the CDH1 promoter.

RESULTS:

Correlations were observed between tumour progression and both nuclear expression of E-cadherin and reduced CDH1 mRNA. The E-cadherin expression was not determined by the methylation pattern of the CDH1 promoter.

CONCLUSIONS:

Corticotroph tumour progression was associated with reduced expression of the epithelial marker E-cadherin.

Place, publisher, year, edition, pages
2011. Vol. 75, no 6, 811-818 p.
National Category
Clinical Laboratory Medicine Basic Medicine
Research subject
Pathology
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URN: urn:nbn:se:uu:diva-189885DOI: 10.1111/j.1365-2265.2011.04109.xPubMedID: 21595730OAI: oai:DiVA.org:uu-189885DiVA: diva2:582532
Available from: 2013-01-04 Created: 2013-01-04 Last updated: 2017-12-06

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