BDNF as otoprotectant in toxin-induced hearing loss
2013 (English)In: Acta Oto-Laryngologica, ISSN 0001-6489, Vol. 133, no 1, 4-11 p.Article in journal (Refereed) Published
Conclusion: Brain-derived neurotrophic factor (BDNF) can prevent auditory brainstem response (ABR) threshold shift changes caused by Pseudomonas aeruginosa exotoxin A (PaExoA). Objective: Peptides of the neurotrophin family are known to prevent neuronal death during embryonic development by interacting with specific membrane receptors. The purpose of this study was to investigate whether a single dose of BDNF is an effective protectant against toxic effects of PaExoA-induced ABR threshold shifts. Materials and Methods: Eight groups of Sprague-Dawley rats were used. There were five control groups (n = 20) as follows. Group A (n = 4) received NaCl solution; group B (n = 3) received 4 mu g BDNF; group C (n = 4) received 1 mu g/20 mu l PaExoA; group D (n = 4) received 2 mu g/20 mu l PaExoA; groupE (n = 5) received 10 mu g/20 mu l PaExoA injected into the round window niche. Three treatment groups (n = 13) received a single dose of PaExoA and 4 mu g of BDNF simultaneously. Group 1 (n = 3) received 1 mu g/20 mu l PaExoA + 4 mu g of BDNF; group 2 (n = 5) received 2 mu g/20 mu l PaExoA + 4 mg BDNF; group 3 (n = 5) received 10 mu g/20 mu l PaExoA+ 4 mu g BDNF. ABR was used to measure efficacy by analyzing threshold shifts before and after injections. Results: A single dose of BDNF prevented changes in ABR thresholds following exposure to increasing concentrations of PaExoA injected into the middle ear.
Place, publisher, year, edition, pages
2013. Vol. 133, no 1, 4-11 p.
Auditory brainstem response, Brain-derived neurotrophic factor, Pseudomonas aeruginosa exotoxin A, cochlea, round window membrane, rat
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-192429DOI: 10.3109/00016489.2012.712216ISI: 000312525800001OAI: oai:DiVA.org:uu-192429DiVA: diva2:600369