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PCR detection of haemophilus influenzae from respiratory specimens
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Bacteriology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Clinical Bacteriology.
2013 (English)In: PCR Detection of Microbial Pathogens / [ed] Mark Wilks, Humana Press, 2013, 2, 115-123 p.Chapter in book (Refereed)
Abstract [en]

The detection of Haemophilus influenzae by conventional methods like culture is time-consuming and may give false-negative results, especially during ongoing antibiotic treatment. Therefore, non-culture based methods that are sensitive, specific, and rapid are valuable for early diagnosis and effective therapy. Here we describe a quantitative real-time PCR assay based on the outer membrane P6 gene omp6, to detect H. influenzae and its application on respiratory tract specimens.

Place, publisher, year, edition, pages
Humana Press, 2013, 2. 115-123 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 943
Keyword [en]
Haemophilus influenzae, Outer membrane P6, Pneumonia, Quantitative PCR, Real-time PCR
National Category
Medical and Health Sciences Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-191997DOI: 10.1007/978-1-60327-353-4_7ISBN: 978-160327-352-7 OAI: oai:DiVA.org:uu-191997DiVA: diva2:600457
Available from: 2013-01-24 Created: 2013-01-15 Last updated: 2013-01-24Bibliographically approved

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Abdeldaim, Guma M. K.Herrmann, Björn

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