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Isoprenylation of plant proteins in vivo: Isoprenylated proteins are abundant in the mitochondria and nuclei of spinach
Arrhenius Laboratories for Natural Sciences, Biochemistry Department, University of Stockholm, Stockholm S-106 91 Sweden.
Arrhenius Laboratories for Natural Sciences, Biochemistry Department, University of Stockholm, Stockholm S-106 91 Sweden.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-532 Warsaw, Poland.
Arrhenius Laboratories for Natural Sciences, Biochemistry Department, University of Stockholm, Stockholm S-106 91 Sweden.
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1995 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 270, no 2, 566-572 p.Article in journal (Refereed) Published
Abstract [en]

Protein isoprenylation in vivo is demonstrated using spinach seedlings labeled with [3H]mevalonate. This report provides evidence for the occurrence of a large number of isoprenylated proteins in plants. Seedlings, without roots, were labeled quantitatively through the cut stem. Mevinolin treatment of the seedlings resulted in increased incorporation of radiolabel into proteins. Approximately 30 labeled bands could be detected after autoradiography of SDS-polyacrylamide gel electrophoresis-separated polypeptides, ranging in molecular mass from 6 to 200 kDa. Methyl iodide hydrolysis resulted in the release of covalently bound farnesol, geranylgeraniol, phytol, and some unidentified isoprenoid compounds from mevalonate-labeled proteins. It was found that all cellular fractions contained some isoprenylated proteins, although most were located in the mitochondria and nuclei. Subfractionation of the nucleus revealed that the majority of isoprenylated proteins in this compartment were components of the nuclear matrix. The results demonstrate that in vivo labeling of a complex organism can be performed using a plant system in order to study protein isoprenylation and distribution of modified proteins in different cellular compartments.

Place, publisher, year, edition, pages
1995. Vol. 270, no 2, 566-572 p.
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
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URN: urn:nbn:se:uu:diva-193081DOI: 10.1074/jbc.270.2.566PubMedID: 7822281OAI: oai:DiVA.org:uu-193081DiVA: diva2:601292
Available from: 2013-01-29 Created: 2013-01-29 Last updated: 2017-12-06Bibliographically approved

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Parmryd, Ingela

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