Chloroplastic prenylated proteins
1997 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 414, no 3, 527-531 p.Article in journal (Refereed) Published
By in vivo [3H]mevalonate labelling of spinach combined with biochemical analysis, evidence is provided for the existence of protein prenylation in chloroplasts. Approximately 20 prenylated polypeptides were resolved by SDS-PAGE followed by autoradiography. Thermolysin treatment of intact chloroplasts revealed that about 40% of the prenylated polypeptides were associated with the cytoplasmic surface of the outer envelope membrane. The remaining portion was present in thylakoids and/or the inner envelope membrane. The majority of the prenylated polypeptides were associated with larger membrane protein complexes. A farnesyl protein transferase activity was found to be associated with the thylakoid membrane.
Place, publisher, year, edition, pages
1997. Vol. 414, no 3, 527-531 p.
chloroplast; farnesyl protein transferase; prenylation
Biochemistry and Molecular Biology
Research subject Biochemistry
IdentifiersURN: urn:nbn:se:uu:diva-193088DOI: 10.1016/S0014-5793(97)01063-6ISI: A1997XX57700008PubMedID: 9323028OAI: oai:DiVA.org:uu-193088DiVA: diva2:601304