uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Selective cell capture and analysis using shallow antibody-coated microchannels
Show others and affiliations
2012 (English)In: Biomicrofluidics, ISSN 1932-1058, Vol. 6, no 4, 044117- p.Article in journal (Refereed) Published
Abstract [en]

Demand for analysis of rare cells such as circulating tumor cells in blood at the single molecule level has recently grown. For this purpose, several cell separation methods based on antibody-coated micropillars have been developed (e.g., Nagrath , Nature 450, 1235-1239 (2007)). However, it is difficult to ensure capture of targeted cells by these methods because capture depends on the probability of cell-micropillar collisions. We developed a new structure that actively exploits cellular flexibility for more efficient capture of a small number of cells in a target area. The depth of the sandwiching channel was slightly smaller than the diameter of the cells to ensure contact with the channel wall. For cell selection, we used anti-epithelial cell adhesion molecule antibodies, which specifically bind epithelial cells. First, we demonstrated cell capture with human promyelocytic leukemia (HL-60) cells, which are relatively homogeneous in size; in situ single molecule analysis was verified by our rolling circle amplification (RCA) method. Then, we used breast cancer cells (SK-BR-3) in blood, and demonstrated selective capture and cancer marker (HER2) detection by RCA. Cell capture by antibody-coated microchannels was greater than with negative control cells (RPMI-1788 lymphocytes) and non-coated microchannels. This system can be used to analyze small numbers of target cells in large quantities of mixed samples.

Place, publisher, year, edition, pages
2012. Vol. 6, no 4, 044117- p.
Keyword [en]
adhesion, biological organs, biomechanics, biomedical equipment, bioMEMS, blood, cancer, cellular biophysics, microchannel flow, molecular biophysics, proteins, tumours
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-193029DOI: 10.1063/1.4771968ISI: 000312831100018OAI: oai:DiVA.org:uu-193029DiVA: diva2:602891
Available from: 2013-02-04 Created: 2013-01-28 Last updated: 2013-02-04Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Nilsson, Mats
By organisation
Molecular tools
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 284 hits
ReferencesLink to record
Permanent link

Direct link