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Tissue factor pathway inhibitor 2 is found in skin and its C-terminal region encodes for antibacterial activity
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2012 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 7, no 12, e52772- p.Article in journal (Refereed) Published
Abstract [en]

BACKGROUND

Tissue factor pathway inhibitor 2 (TFPI-2) is a matrix-associated serine protease inhibitor with an enigmatic function in vivo. Here, we describe that TFPI-2 is present in fibrin of wounds and also expressed in skin, where it is up-regulated upon wounding.

METHODOLOGY AND PRINCIPAL FINDINGS

Neutrophil elastase cleaved TFPI-2, and a C-terminal fragment was found to bind to bacteria. Similarly, a prototypic peptide representing this C-terminal part, EDC34, bound to bacteria and bacterial lipopolysaccharide, and induced bacterial permeabilization. The peptide also induced leakage in artificial liposomes, and displayed a random coil conformation upon interactions with liposomes as well as lipopolysaccharide. EDC34 was antibacterial against both Gram-negative and Gram-positive bacteria in physiological buffer conditions.

CONCLUSIONS/SIGNIFICANCE

The results demonstrate that the C-terminus of TFPI-2 encodes for antimicrobial activity, and may be released during wounding.

Place, publisher, year, edition, pages
2012. Vol. 7, no 12, e52772- p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-193848DOI: 10.1371/journal.pone.0052772ISI: 000313618800143PubMedID: 23300768OAI: oai:DiVA.org:uu-193848DiVA: diva2:603592
Available from: 2013-02-06 Created: 2013-02-06 Last updated: 2017-12-06Bibliographically approved

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Malmsten, Martin

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