Accelerator mass spectrometry of ultra-small samples with applications in the biosciences
2013 (English)In: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, ISSN 0168-583X, Vol. 294, 97-103 p.Article in journal, Meeting abstract (Refereed) Published
An overview is presented covering the biological accelerator mass spectrometry activities at Uppsala University. The research utilizes the Uppsala University Tandem laboratory facilities, including a 5 MV Pelletron tandem accelerator and two stable isotope ratio mass spectrometers. In addition, a dedicated sample preparation laboratory for biological samples with natural activity is in use, as well as another laboratory specifically for C-14-labeled samples. A variety of ongoing projects are described and presented. Examples are: (1) Ultra-small sample AMS. We routinely analyze samples with masses in the 5-10 mu g C range. Data is presented regarding the sample preparation method, (2) bomb peak biological dating of ultra-small samples. A long term project is presented where purified and cell-specific DNA from various part of the human body including the heart and the brain are analyzed with the aim of extracting regeneration rate of the various human cells, (3) biological dating of various human biopsies, including atherosclerosis related plaques is presented. The average built up time of the surgically removed human carotid plaques have been measured and correlated to various data including the level of insulin in the human blood, and (4) In addition to standard microdosing type measurements using small pharmaceutical drugs, pre-clinical pharmacokinetic data from a macromolecular drug candidate are discussed.
Place, publisher, year, edition, pages
2013. Vol. 294, 97-103 p.
IdentifiersURN: urn:nbn:se:uu:diva-194211DOI: 10.1016/j.nimb.2012.08.054ISI: 000313234300019OAI: oai:DiVA.org:uu-194211DiVA: diva2:605173
12th International Conference on Accelerator Mass Spectrometry (AMS), 20-25 March 2011, Wellington, NEW ZEALAND