uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Guiding differentiation of stem cells in vivo by tetracycline-controlled expression of key transcription factors
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. (Neuroanatomi)
Unviersity of Erlangen-Nuremberg. (Microbiology)
2012 (English)In: Cell Transplantation, ISSN 0963-6897, E-ISSN 1555-3892, Vol. 21, 2537-2554 p.Article, review/survey (Refereed) Published
Abstract [en]

Transplantation of stem or progenitor cells is an attractive strategy for cell replacement therapy. However, poor long-term survival and insufficiently reproducible differentiation to functionally appropriate cells in vivo still present major obstacles for translation of this methodology to clinical applications. Numerous experimental studies have revealed that the expression of just a few transcription factors can be sufficient to drive stem cell differentiation towards a specific cell type, to transdifferentiate cells from one fate to another, or to dedifferentiate mature cells to pluripotent stem/progenitor cells (iPSCs). We thus propose here to apply the strategy of expressing the relevant key transcription factors to guide the differentiation of transplanted cells to the desired cell fate in vivo. To achieve this requires tools allowing us to control the expression of these genes in the transplant. Here, we describe drug-inducible systems that allow us to sequentially and timely activate gene expression from the outside, with a particular emphasis on the Tet system which has been widely and successfully used in stem cells. These regulatory systems offer a tool for strictly limiting gene expression to the respective optimal stage after transplantation. This approach will direct the differentiation of the immature stem/progenitor cells in vivo to the desired cell type.

Place, publisher, year, edition, pages
2012. Vol. 21, 2537-2554 p.
Keyword [en]
Cell survival; Central nervous system; Gene regulation
National Category
Neurosciences
Research subject
Neuroscience
Identifiers
URN: urn:nbn:se:uu:diva-194505DOI: 10.3727/096368911X637407ISI: 000315001400001OAI: oai:DiVA.org:uu-194505DiVA: diva2:605636
Available from: 2013-02-14 Created: 2013-02-14 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

Cell transplantation review(305 kB)507 downloads
File information
File name FULLTEXT01.pdfFile size 305 kBChecksum SHA-512
eb58154badad1713b47b3510e33c9eebdbb8336ac8f615d79bab2bbfff81c448e0e129525662a30731b6a6c2658f02a4ee688d3d22fe7fa999c5d07890def08b
Type fulltextMimetype application/pdf

Other links

Publisher's full texthttp://www.ingentaconnect.com/search/download;jsessionid=3ptmv6iopoaga.alice?pub=infobike%3a%2f%2fcog%2fct%2f2012%2f00000021%2f00000012%2fart00001&mimetype=text%2fhtml

Authority records BETA

Kozlova, Elena

Search in DiVA

By author/editor
Kozlova, Elena
By organisation
Department of Neuroscience
In the same journal
Cell Transplantation
Neurosciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 507 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 680 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf