Characterization of β-amyloid and Inflammatory processes with age in Tg2576 Alzheimer mice brain
Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Recent molecular imaging has advanced our knowledge of β-amyloid (Aβ) and neuroinflammatory processes in living patients with Alzheimer’s disease (AD), using tracers such as 11C-PIB, 11C-PK11195 as well as 11C-Deprenyl. In this study, I aimed to investigate thoroughly the distribution of Aβ pathology in relation to neuroinflammatory processes and cholinergic neurotransmitter receptor subtypes in the cortex and hippocampus of 4-22 month-old Tg2576 mice. Triple immunohistochemistry was performed to visualize Aβ42, GFAP (a marker of activated astrocytes), Iba1 (a marker of activated microglia) and α7-nicotinic receptors (nAChRs). Different types of Aβ42 deposits; cored/diffused plaques, fleecy and granular deposits as well as GFAP+ astrocytes (Aβ-plaque associated and non-associated) were characterized in mice based on classification criteria described previously in AD postmortem brain. Aβ42 immunoreactivity showed an age dependent increase. Granular deposits were present in both the cortex and hippocampus of 4 and 8-13 months old mice, whereas cored plaques were only detected in the cortex of 18-22 months old mice. GFAP+ immunoreactivity demonstrated an age dependent increase and Aβ plaque non-associated astrocytes were abundant in the cortex and within the hippocampus of mice at 18-22 months of age. Interestingly the expression of α7-nAChRs on activated astrocytes was only detected in the cortex of 18-22 months old mice. Iba1+ microglia showed an age dependent increase but no expression of α7-nAChRs was detected on activated microglia. In conclusion, Aβ42, activated microglia and astrocytes all show an age dependent increase, but with different distributions and time course in Tg2576 mice brain regions, as well as regional alterations in α7-nAChR expression on activated astrocytes.
Place, publisher, year, edition, pages
2013. , 35 p.
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-194651OAI: oai:DiVA.org:uu-194651DiVA: diva2:606615
Subject / course
Master of Science Programme in Pharmacy
Voytenko, Larysa, Ni, Ruiqing, & Marutle, Amelia