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Multiplex protein detection with DNA readout via mass spectrometry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2013 (English)In: New Biotechnology, ISSN 1871-6784, E-ISSN 1876-4347, Vol. 30, no 2, 153-158 p.Article in journal (Refereed) Published
Abstract [en]

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody-oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

Place, publisher, year, edition, pages
2013. Vol. 30, no 2, 153-158 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-194912DOI: 10.1016/j.nbt.2012.11.003ISI: 000313786400008OAI: oai:DiVA.org:uu-194912DiVA: diva2:606651
Note

De 2 första författarna delar förstaförfattarskapet

Available from: 2013-02-20 Created: 2013-02-19 Last updated: 2017-12-06Bibliographically approved

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Kamali-Moghaddam, MasoodLandegren, Ulf

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Molecular toolsScience for Life Laboratory, SciLifeLabDepartment of Immunology, Genetics and Pathology
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