Screening of herpes simplex virus type 1 isolates for acyclovir resistance using DiviTum® assay
2013 (English)In: Journal of Virological Methods, ISSN 0166-0934, E-ISSN 1879-0984, Vol. 188, no 1-2, 70-72 p.Article in journal (Refereed) Published
Rapid alternative methods are required to evaluate easily acyclovir (ACV) sensitivity of clinical herpes simplex virus (HSV) isolates. The objective of this study was to screen 54 ACV-sensitive and 41 ACV-resistant clinical HSV-1 isolates, well characterized by phenotypic and genotypic methods, for the phosphorylation activity of the viral thymidine kinase (TK) using a commercially available and modified non-radioactive DiviTum® test on the basis of an indirect enzyme linked immunosorbent assay. The ACV-sensitive HSV-1 isolates had high TK activity values between 31.5±6.4 DiviTum® Units per liter (DU/L) and 487.4±60.1DU/L. The mean activity of all ACV-sensitive isolates was calculated as 212.3±15.7 DU/L. By contrast, the mean activity of all ACV-resistant HSV-1 isolates was significantly lower at 5.5±1.3DU/L. Out of the 41 ACV-resistant HSV-1 isolates, 38 had no or very low phosphorylation activities of the viral TK between 0DU/L and 9.3±3.2DU/L. The remaining three ACV-resistant viral isolates had TK activities between 44.6±5.1DU/L and 80.9±13.3DU/L. In conclusion, the modified DiviTum® test can be used to screen HSV-1 isolates for their sensitivity to ACV. Acyclovir-sensitive HSV-1 isolates show TK activities >30DU/L and ACV-resistant isolates have activity values <10DU/L. However, single ACV-resistant HSV-1 isolates can have TK activity values >30DU/L. These strains are most likely ACV-resistant TK-altered mutants, but no evidence was provided for an alteration of the TK.
Place, publisher, year, edition, pages
2013. Vol. 188, no 1-2, 70-72 p.
Acyclovir resistance, HSV-1, Phosphorylation activity, Thymidine kinase
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-194841DOI: 10.1016/j.jviromet.2012.12.001OAI: oai:DiVA.org:uu-194841DiVA: diva2:606767