uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Equine arteritis virus induced cell death is associated with activation of the intrinsic apoptotic signalling pathway
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
Unit for Virology, Department of Virology, Immunobiology and Parasitology of the National Veterinary Institute (SVA).
Show others and affiliations
2013 (English)In: Virus Research, ISSN 0168-1702, E-ISSN 1872-7492, Vol. 171, no 1, 222-226 p.Article in journal (Refereed) Published
Abstract [en]

Equine arteritis virus (EAV) causes a respiratory and reproductive disease in horses, equine viral arteritis. Though cell death in infection with EAV is considered to occur by apoptosis, the underlying molecular mechanism has not been extensively elucidated. We investigated the expression of mRNA of pro-apoptotic and caspase genes during EAV infection in BHK21 cells, a well-established cell type for EAV replication. Using a SYBR Green real-time PCR, mRNA of p53, Bax, caspase 3 and caspase 9 were found up-regulated in a time dependent manner in EAV infected cells. Western blot analysis for caspase 3 and caspase 9 showed expression of cleaved forms of these proteins during EAV infection. In addition, a luminescence-based cell assay for caspase 3/7 activation as a hallmark in apoptosis confirmed apoptotic cell death. The findings demonstrate that cell death in EAV infected BHK21 cells results from apoptosis mediated through the intrinsic signalling pathway.

Place, publisher, year, edition, pages
2013. Vol. 171, no 1, 222-226 p.
Keyword [en]
3/7, Apoptosis, Bax, Caspase 9, Equine arteritis virus, P53, Real-time PCR
National Category
Infectious Medicine
URN: urn:nbn:se:uu:diva-195151DOI: 10.1016/j.virusres.2012.10.004ISI: 000315607300026OAI: oai:DiVA.org:uu-195151DiVA: diva2:607348
Available from: 2013-02-22 Created: 2013-02-21 Last updated: 2014-11-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Paidikondala, Maruthibabu
By organisation
Biology Education Centre
In the same journal
Virus Research
Infectious Medicine

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 199 hits
ReferencesLink to record
Permanent link

Direct link