The fungus Cunninghamella elegans can produce human and equine metabolites of selective androgen receptor modulators (SARMs)
2013 (English)In: Xenobiotica, ISSN 0049-8254, E-ISSN 1366-5928, Vol. 43, no 5, 409-420 p.Article in journal (Refereed) Published
1. Selective androgen receptor modulators (SARMs) are a group of substances that have potential to be used as doping agents in sports. Being a relatively new group not available on the open market means that no reference materials are commercially available for the main metabolites. In the presented study, the in vitro metabolism of SARMs by the fungus Cunninghamella elegans has been investigated with the purpose of finding out if it can produce relevant human and equine metabolites.
2. Three different SARMs, S1, S4 and S24, were incubated for 5 days with C. elegans. The samples were analysed both with and without sample pretreatment using ultra performance liquid chromatography coupled to high resolution mass spectrometry.
3. All the important phase I and some phase II metabolites from human and horse were formed by the fungus. They were formed through reactions such as hydroxylation, deacetylation, O-dephenylation, nitro-reduction, acetylation and sulfonation.
4. The study showed that the fungus produced relevant metabolites of the SARMs and thus can be used to mimic mammalian metabolism. Furthermore, it has the potential to be used for future production of reference material.
Place, publisher, year, edition, pages
2013. Vol. 43, no 5, 409-420 p.
Pharmaceutical Sciences Medicinal Chemistry Analytical Chemistry
IdentifiersURN: urn:nbn:se:uu:diva-196318DOI: 10.3109/00498254.2012.729102ISI: 000316952100002OAI: oai:DiVA.org:uu-196318DiVA: diva2:609805
MEDLINE AN 2013135005(Journal; Article; (JOURNAL ARTICLE))2013-03-072013-03-072014-04-29Bibliographically approved