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Characterization of RISC-associated adenoviral small RNAs
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
2011 (English)In: Methods in molecular biology (Clifton, N.J.), ISSN 1940-6029, Vol. 721, 183-198 p.Article, review/survey (Refereed) Published
Abstract [en]

RNA interference (RNAi) plays novel roles in both host antiviral defense and viral replication. It has been shown that some viruses can exploit the RNAi machinery for their own benefit by encoding for their own viral small RNAs. Here we present a collection of methods to study adenoviral small RNAs, specifically a method for immunopurification of RNA-induced silencing complex (RISC) and a biochemical assay for the activity of purified RISC associated with adenoviral small RNAs.

Place, publisher, year, edition, pages
2011. Vol. 721, 183-198 p.
National Category
Cell and Molecular Biology
URN: urn:nbn:se:uu:diva-196712DOI: 10.1007/978-1-61779-037-9_11PubMedID: 21431686OAI: oai:DiVA.org:uu-196712DiVA: diva2:610838
Available from: 2013-03-13 Created: 2013-03-13 Last updated: 2013-08-20Bibliographically approved

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Akusjärvi, Göran
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Department of Medical Biochemistry and Microbiology
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