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Protein kinase a-dependent phosphorylation of serine 119 in the proto-oncogenic serine/arginine-rich splicing factor 1 modulates its activity as a splicing enhancer protein.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2011 (English)In: Genes & cancer, ISSN 1947-6027, Vol. 2, no 8, 841-851 p.Article in journal (Refereed) Published
Abstract [en]

Serine/arginine-rich splicing factor 1 (SRSF1), previously designated SF2/ASF, belongs to a family of SR proteins that regulate constitutive and alternative splicing. SRSF1 expression is increased in tumors from several tissues and elicits changes in key target genes involved in tumor genesis. Several protein kinases phosphorylate SRSF1, which regulates its localization and function. It is previously reported that protein kinase A (PKA) phosphorylates SRSF1, but the importance of this modification is not well characterized. Here, we show that PKA phosphorylates SRSF1 on serine 119 in vitro. Phosphorylation of SRSF1 on this site enhanced the RNA binding capacity of SRSF1 in vivo and reduced the protein's capacity to activate splicing of the Minx transcript in vitro. We also confirm an interaction between SRSF1 and PKA Cα1 and demonstrate that this interaction is not dependent on serine 119 phosphorylation but requires active PKA Cα1. We conclude that PKA phosphorylation of SRSF1 at serine 119 regulates SFRS1-dependent RNA binding and processing but not its interaction with PKA.

Place, publisher, year, edition, pages
2011. Vol. 2, no 8, 841-851 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-196713DOI: 10.1177/1947601911430226PubMedID: 22393468OAI: oai:DiVA.org:uu-196713DiVA: diva2:610839
Available from: 2013-03-13 Created: 2013-03-13 Last updated: 2013-03-13Bibliographically approved

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Akusjärvi, Göran
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Department of Medical Biochemistry and Microbiology
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