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Production and cleavage specificity determination of serine proteases mMCP-4, mMCP-5, rMCP-2 and two platypus serine proteases of the chymase locus.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Serine proteases are a family of enzymes with a wide array of functions across both eukaryotes and prokaryotes. Here we have attempted to produce the serine proteases rat mast cell protease 2 and mouse mast cell protease 5 in a culture of HEK 293 cells; and mouse mast cell protease 4, platypus granzyme B-like protease and platypus hypothetical protease in a baculovirus expression system. Following production we wanted to analyse these serine proteases using a phage display assay and a battery of chromogenic substrates.

Place, publisher, year, edition, pages
2013. , 55 p.
Keyword [en]
Serine protease, substrate, hek 293, baculovirus, phage display, chromogenic substrates
National Category
URN: urn:nbn:se:uu:diva-197088OAI: oai:DiVA.org:uu-197088DiVA: diva2:611640
Educational program
Molecular Biotechnology Engineering Programme
Life Earth Science
Available from: 2013-03-18 Created: 2013-03-18 Last updated: 2013-03-18Bibliographically approved

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Sidibeh, Cherno Omar
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Biology Education Centre

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