uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Production and cleavage specificity determination of serine proteases mMCP-4, mMCP-5, rMCP-2 and two platypus serine proteases of the chymase locus.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Serine proteases are a family of enzymes with a wide array of functions across both eukaryotes and prokaryotes. Here we have attempted to produce the serine proteases rat mast cell protease 2 and mouse mast cell protease 5 in a culture of HEK 293 cells; and mouse mast cell protease 4, platypus granzyme B-like protease and platypus hypothetical protease in a baculovirus expression system. Following production we wanted to analyse these serine proteases using a phage display assay and a battery of chromogenic substrates.

Place, publisher, year, edition, pages
2013. , 55 p.
Keyword [en]
Serine protease, substrate, hek 293, baculovirus, phage display, chromogenic substrates
National Category
Immunology
Identifiers
URN: urn:nbn:se:uu:diva-197088OAI: oai:DiVA.org:uu-197088DiVA: diva2:611640
Educational program
Molecular Biotechnology Engineering Programme
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2013-03-18 Created: 2013-03-18 Last updated: 2013-03-18Bibliographically approved

Open Access in DiVA

fulltext(2618 kB)48 downloads
File information
File name FULLTEXT01.pdfFile size 2618 kBChecksum SHA-512
a66494f9617448a54067073c956ebe670bf1e6ff8ae48ffabedd2aa63c5dc022a37c3c72742582f000faecb9acd17f4406ea18268af8a995e11e3ea59026883b
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Sidibeh, Cherno Omar
By organisation
Biology Education Centre
Immunology

Search outside of DiVA

GoogleGoogle Scholar
Total: 48 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 412 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf