Bacteria viability assessment after photocatalytic treatment
2014 (English)In: 3 Biotech, ISSN 2190-5738, Vol. 4, no 2, 149-157 p.Article in journal (Refereed) Published
The aim of the present work was to evaluate several methods for analyzing the viability of bacteria after antibacterial photocatalytic treatment. Colony-forming unit (CFU) counting, metabolic activity assays based on resazurin and phenol red and the Live/Dead® BacLight™ bacterial viability assay (Live/Dead staining) were employed to assess photocatalytically treated Staphylococcus epidermidis and Streptococcus mutans. The results showed conformity between CFU counting and the metabolic activity assays, while Live/Dead staining showed a significantly higher viability post-treatment. This indicates that the Live/Dead staining test may not be suitable for assessing bacterial viability after photocatalytic treatment and that, in general, care should be taken when selecting a method for determining the viability of bacteria subjected to photocatalysis. The present findings are expected to become valuable for the development and evaluation of photocatalytically based disinfection applications
Place, publisher, year, edition, pages
Springer, 2014. Vol. 4, no 2, 149-157 p.
Engineering and Technology
Research subject Engineering Science with specialization in Nanotechnology and Functional Materials
IdentifiersURN: urn:nbn:se:uu:diva-197796DOI: 10.1007/s13205-013-0137-1OAI: oai:DiVA.org:uu-197796DiVA: diva2:614266