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Photocatalysis induces bioactivity of an organic polymer based material
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.ORCID iD: 0000-0002-5496-9664
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
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2014 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 4, no 101, 57715-57723 p.Article in journal (Refereed) Published
Abstract [en]

Several materials, like bioglasses, sintered hydroxyapatite and Ti metals and alloys, have the ability to bond to living bone in vivo, which is a desirable property of biomaterials called bioactivity. In this work, we present a novel strategy to develop bioactivity on the non-bioactive surface of a resin-TiO2 nanocomposite through photocatalysis. The results show that UV irradiation (365 nm, 10 mW cm(-2)) for 8 to 16 h on the resin-TiO2 nanocomposite immersed in water induces bioactivity as indicated by hydroxyapatite growth following immersion of the samples in Dulbecco's phosphate buffered saline for 7 days at 37 degrees C. While a nonirradiated resin-TiO2 surface did not show any hydroxyapatite deposition, a surface after 16 h of UV irradiation was fully covered by hydroxyapatite. In vitro cell adhesion of osteoblast-like MG63 cells confirmed the biocompatibility and bioactivity of the resin-TiO2 surfaces with a hydroxyapatite deposition layer, while the non-irradiated resin-TiO2 surface showed no cell adhesion. Resin-TiO2 nanocomposites, with or without UV irradiation, proved to be nontoxic to two human cell lines, human dermal fibroblasts (hDF) and MG63 cells. It was also shown that an increased dose of UV irradiation decreased bacterial adhesion, which is an additional benefit of the UV treatment and a favourable property for biomedical applications. The combined benefits of biocompatibility, bioactivity, decreased bacterial adhesion and the highly efficient disinfection property of TiO2 photocatalysis under UV light make this resin-TiO2 material an interesting candidate for implant and biomedical device applications.

Place, publisher, year, edition, pages
2014. Vol. 4, no 101, 57715-57723 p.
National Category
Materials Engineering
Identifiers
URN: urn:nbn:se:uu:diva-197798DOI: 10.1039/c4ra08805kISI: 000345651600048OAI: oai:DiVA.org:uu-197798DiVA: diva2:614268
Available from: 2013-04-04 Created: 2013-04-04 Last updated: 2017-12-06Bibliographically approved
In thesis
1. Titanium Dioxide Photocatalysis in Biomaterials Applications
Open this publication in new window or tab >>Titanium Dioxide Photocatalysis in Biomaterials Applications
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Despite extensive preventative efforts, the problem of controlling infections associated with biomedical materials persists. Bacteria tend to colonize on biocompatible materials and form biofilms; thus, novel biomaterials with antibacterial properties are of great interest. In this thesis, titanium dioxide (TiO2)-associated photocatalysis under ultraviolet (UV) irradiation was investigated as a strategy for developing bioactivity and antibacterial properties on biomaterials. Although much of the work was specifically directed towards dental materials, the results presented are applicable to a wide range of biomaterial applications.

Most of the experimental work in the thesis was based on a resin-TiO2 nanocomposite that was prepared by adding 20 wt% TiO2 nanoparticles to a resin-based polymer material. Tests showed that the addition of the nanoparticles endowed the adhesive material with photocatalytic activity without affecting the functional bonding strength. Subsequent studies indicated a number of additional beneficial properties associated with the nanocomposite that appear promising for biomaterial applications. For example, irradiation with UV light induced bioactivity on the otherwise non-bioactive nanocomposite; this was indicated by hydroxyapatite formation on the surface following soaking in Dulbecco’s phosphate-buffered saline. Under UV irradiation, the resin-TiO2 nanocomposite provided effective antibacterial action against both planktonic and biofilm bacteria. UV irradiation of the nanocomposite also provided a prolonged antibacterial effect that continued after removal of the UV light source. UV treatment also reduced bacterial adhesion to the resin-TiO2 surface.

The mechanisms involved in the antibacterial effects of TiO2 photocatalysis were studied by investigating the specific contributions of the photocatalytic reaction products (the reactive oxygen species) and their disinfection kinetics. Methods of improving the viability analysis of bacteria subjected to photocatalysis were also developed. 

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. 57 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1033
Keyword
titanium dioxide, photocatalysis, bioactivity, antibacterial effect, metabolic activity assay, biofilm, reactive oxygen species, disinfection kinetics, post-UV
National Category
Nano Technology
Identifiers
urn:nbn:se:uu:diva-160634 (URN)978-91-554-8634-1 (ISBN)
Public defence
2013-05-22, Häggsalen, Ångström laboratory, Lägerhyddsvägen 1, Uppsala, 13:30 (English)
Supervisors
Available from: 2013-04-26 Created: 2011-10-27 Last updated: 2013-08-30Bibliographically approved

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Cai, YanlingStrömme, MariaZhang, PengEngqvist, HåkanWelch, Ken

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