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A generic capture assay for immunogenicity, using Biacore
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

The purpose of this investigation was to create and optimise a capture assay for the detectionof anti-drug antibodies (ADA) in human plasma, using Biacore. We also dealt with the nonspecificplasma binding to mouse-derived anti-biotin which may occur in the capture assay.By paying attention to these things we aimed at reaching as high sensitivity as possible for theADA detection.

The capture assay also benefited and gained flexibility from using the same regenerationsolution irrespective of drug and from having a composition that minimises the risk ofdamaging drug epitopes.

Place, publisher, year, edition, pages
2013. , 40 p.
Keyword [en]
Immunogenicity, immunoassay, capture assay, Biacore, non-specific binding, anti-drug antibodies (ADA), human plasma, anti-biotin, biotinylation
National Category
Biochemistry and Molecular Biology
URN: urn:nbn:se:uu:diva-198767OAI: oai:DiVA.org:uu-198767DiVA: diva2:617838
Educational program
Molecular Biotechnology Engineering Programme
Available from: 2013-04-24 Created: 2013-04-24 Last updated: 2013-04-24Bibliographically approved

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