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Unfolding probes: A novel method for biomolecular detection
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Unfolding probes is a novel detection method for biomolecules, involving an oligonucleotide probe that can be amplified and which thereby gives rise to a strong signal for individual probe - target interactions. The present project focuses on a variant of unfolding probes called 2-fold probes and on optimizing the detection efficiency of synthetic targets. Furthermore, proof of concept experiments were performed for the detection of Her2 mRNA in cells.

Place, publisher, year, edition, pages
2013. , 32 p.
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-198831OAI: oai:DiVA.org:uu-198831DiVA: diva2:618128
Educational program
Molecular Biotechnology Engineering Programme
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2013-04-26 Created: 2013-04-25 Last updated: 2013-04-26Bibliographically approved

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fulltext(1538 kB)82 downloads
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CiteExportLink to record
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