Identification and verification of microRNAs by high-throughput sequencing
2013 (English)In: Dictyostelium discoideum Protocols / [ed] Ludwig Eichinger and Francisco Rivero, Humana Press, 2013, 2, Vol. 983, 125-138 p.Chapter in book (Other academic)
High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.
Place, publisher, year, edition, pages
Humana Press, 2013, 2. Vol. 983, 125-138 p.
, Methods in Molecular Biology, ISSN 1063-3745 ; 983
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-198959DOI: 10.1007/978-1-62703-302-2_7PubMedID: 23494305OAI: oai:DiVA.org:uu-198959DiVA: diva2:618774