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Asymmetric growth and division in Mycobacterium spp.: compensatory mechanisms for non-medial septa
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Chemical Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Cell and Molecular Biology, Chemical Biology.
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2013 (English)In: Molecular Microbiology, ISSN 0950-382X, E-ISSN 1365-2958, Vol. 88, no 1, 64-76 p.Article in journal (Refereed) Published
Abstract [en]

Mycobacterium spp., rod-shaped cells belonging to the phylum Actinomycetes, lack the Min- and Noc/Slm systems responsible for preventing the placement of division sites at the poles or over the nucleoids to ensure septal assembly at mid-cell. We show that the position for establishment of the FtsZ-ring in exponentially growing Mycobacterium marinum and Mycobacterium smegmatis cells is nearly random, and that the cells often divide non-medially, producing two unequal but viable daughters. Septal sites and cellular growth disclosed by staining with the membrane-specific dye FM4-64 and fluorescent antibiotic vancomycin (FL-Vanco), respectively, showed that many division sites were off-centre, often over the nucleoids, and that apical cell growth was frequently unequal at the two poles. DNA transfer through the division septum was detected, and translocation activity was supported by the presence of a putative mycobacterial DNA translocase (MSMEG2690) at the majority of the division sites. Time-lapse imaging of single live cells through several generations confirmed both acentric division site placement and unequal polar growth in mycobacteria. Our evidence suggests that post-septal DNA transport and unequal polar growth may compensate for the non-medial division site placement in Mycobacterium spp.

Place, publisher, year, edition, pages
2013. Vol. 88, no 1, 64-76 p.
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Medical and Health Sciences Natural Sciences
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URN: urn:nbn:se:uu:diva-199459DOI: 10.1111/mmi.12169ISI: 000316812100006OAI: oai:DiVA.org:uu-199459DiVA: diva2:620329
Available from: 2013-05-08 Created: 2013-05-06 Last updated: 2017-12-06Bibliographically approved

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Nitharwal, Ram GopalPettersson, B. M. FredrikKirsebom, Leif A.Dasgupta, Santanu

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