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Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2013 (English)In: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 3, 1638- p.Article in journal (Refereed) Published
Abstract [en]

Single molecule methods have provided a significantly new look at the behavior of biomolecules in both equilibrium and non-equilibrium conditions. Most notable are the stretching experiments performed by atomic force microscopes and laser tweezers. Here we present an alternative single molecule method that can unfold a protein domain, observed at electric fields greater than 106 V/m, and is fully controllable by the application of increasing voltages across the membrane of the pore. Furthermore this unfolding mechanism is characterized by measuring both the residence time of the protein within the nanopore and the current blockade. The unfolding data supports a gradual unfolding mechanism rather than the cooperative transition observed by classical urea denaturation experiments. Lastly it is shown that the voltage-mediated unfolding is a function of the stability of the protein by comparing two mutationally destabilized variants of the protein.

Place, publisher, year, edition, pages
2013. Vol. 3, 1638- p.
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Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-199702DOI: 10.1038/srep01638ISI: 000317332400003OAI: oai:DiVA.org:uu-199702DiVA: diva2:621280
Available from: 2013-05-14 Created: 2013-05-13 Last updated: 2017-12-06Bibliographically approved

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