uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Simultaneous determination of renal plasma flow and glomerular filtration rate in conscious mice using dual bolus injection
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Cell Biology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences. (Translational PKPD Research Group)
2013 (English)In: Journal of pharmacological and toxicological methods, ISSN 1056-8719, E-ISSN 1873-488X, Vol. 67, no 3, 187-193 p.Article in journal (Refereed) Published
Abstract [en]

Introduction: The present report describes and evaluates a simple protocol for serial measurements of glomerular filtration rate (GFR) and renal plasma flow (RPF) in conscious mice. Methods: In conscious mice, a bolus of [H-3] methoxy-inulin and [C-14] para-amino-hippuric (PAH) was injected in the tail vein whereupon eight blood samples were taken during the following 75 min. Plasma concentrations were determined by liquid scintillation and clearances of the injected markers were calculated by non-compartmental pharmacokinetic data analysis of the plasma disappearance curves. In anaesthetized mice, the renal extraction ratio of PAH was determined by infusion of PAH and subsequent analysis of blood taken from the carotid artery and the renal vein. The acquired value (0.70 +/- 0.02) was used for all subsequent calculations of RPF. To evaluate the protocol, a crossover study was performed where either the vehicle or the angiotensin II AT1 receptor antagonist candesartan was given prior to the clearance measurements. Results: Baseline values of GFR and RPF were in line with those earlier reported in mice. Administration of candesartan increased RPF and reduced the filtration fraction, whereas GFR was unaltered. These changes are supported by earlier findings and demonstrate that GFR and RPF can be determined independently. Furthermore, modelling experiments demonstrated that acceptable results are obtained even if the number of blood samples is reduced to four which is a way to further simplify the procedure. Discussion: The method provides an effective way for repeated measurements of GFR and RPF in mice without potentially confounding effects of anaesthesia.

Place, publisher, year, edition, pages
2013. Vol. 67, no 3, 187-193 p.
Keyword [en]
Angiotensin II, AT1 receptor, Conscious, Filtration fraction, Glomerular filtration rate, Renal plasma flow
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-200341DOI: 10.1016/j.vascn.2013.01.010ISI: 000317818800009OAI: oai:DiVA.org:uu-200341DiVA: diva2:623631
Available from: 2013-05-28 Created: 2013-05-27 Last updated: 2013-05-28Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Medical Cell BiologyDepartment of Pharmaceutical Biosciences
In the same journal
Journal of pharmacological and toxicological methods
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 132 hits
ReferencesLink to record
Permanent link

Direct link