Bead-based padlock rolling circle amplification for single DNA molecule counting
2013 (English)In: Analytical Biochemistry, ISSN 0003-2697, E-ISSN 1096-0309, Vol. 437, no 1, 43-45 p.Article in journal (Refereed) Published
Padlock rolling circle amplification (RCA) is a powerful analytical method for ultrasensitive DNA detection. Although there are some advantages to bead-based RCA, a detailed study of the relationship between the bead material and the efficiency of bead-based RCA has not been reported. Here, we compared the reaction efficiencies of bead-based RCA performed on two types of bead material: agarose and polystyrene. Agarose was a more suitable material for on-bead RCA. The calibration curve showed linearity between 0.05 and 1 nM, and the limit of detection was 9 pM (9 amol) for Salmonella DNA determination.
Place, publisher, year, edition, pages
2013. Vol. 437, no 1, 43-45 p.
Padlock probe, Rolling circle amplification, Microbeads
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-200645DOI: 10.1016/j.ab.2013.02.016ISI: 000318055300009OAI: oai:DiVA.org:uu-200645DiVA: diva2:625060