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Analysis of the response of Lactococcus lactis towards sublethal alcohol concentrations
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

In this study, I analyzed the Lactococcus lactis subspecies cremoris MG1363 stress response at sub-lethal alcohol levels during exponential growth phase at transcriptomics, proteomics, metabolomics levels. Ethanol, 1-butanol, 1-hexanol were the selected alcohols. Manganese- transporter- and arginine catabolic pathway genes were up-regulated by all alcohols suggesting they evoked oxidative and acidic stress. ATP manganese transporter genes, histidine- and galactose genes were also up-regulated. Purine- and pyrimidine synthesis genes were down-regulated. HPLC analysis displayed decreased biomass yield and glycolytic flux, suggesting increased glycolytic energy production and slowed down overall enzymatic rate. Proteomics analysis displayed differential expressed proteins associated with heat and oxidative stress.

Place, publisher, year, edition, pages
2013. , 65 p.
Series
UPTEC X, 13 006
Keyword [en]
L. lactis, growth experiments, alcohol stress, transcriptomics, proteomics
National Category
Genetics Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-201442OAI: oai:DiVA.org:uu-201442DiVA: diva2:627287
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2013-06-11 Created: 2013-06-11 Last updated: 2013-06-11Bibliographically approved

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CiteExportLink to record
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Citation style
  • apa
  • ieee
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Language
  • de-DE
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  • en-US
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Output format
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