Extraction and quantification of phosphorus derived from DNA and lipids in environmental samples
2013 (English)In: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 115, 336-341 p.Article in journal (Refereed) Published
Understanding the flux and turnover of phosphorus (P) in the environment is important due to the key role P plays in eutrophication and in the ambition to find cost-effective measures to mitigate it. Orthophosphate diesters, including DNA and phospholipids (PLs), represent a potentially degradable P pool that could support future primary production and eutrophication. In this study, extraction techniques were optimized and combined with colorimetric determination of extracted P to provide a selective quantification method for DNA-P and PL-P in agricultural soil, sediment and composted manure. The proposed method is rapid and reproducible with an RSD of <10%. Recovery, evaluated by spiking the sample matrices with DNA and PL standards, was over 95% for both DNA and PLs. The method can be used for the determination of the pool size of the two organic P fractions. Results show that DNA-P comprises 3.0% by weight of the total P (TP) content in the studied soil, 10.4% in the sediment and 8.4% in the compost samples. The values for PL-P are 0.5%, 6.0% and 1.7% for soil, sediment and compost, respectively.
Place, publisher, year, edition, pages
2013. Vol. 115, 336-341 p.
Organic phosphorus, Extraction, Soil, Sediment, Compost
IdentifiersURN: urn:nbn:se:uu:diva-201968DOI: 10.1016/j.talanta.2013.05.042ISI: 000328095600047OAI: oai:DiVA.org:uu-201968DiVA: diva2:630253