Tetrahydrofuran Freeze-Substituted Pancreas Analysed by Nuclear Microscopy and X-ray Microanalysis
1996 (English)In: Journal of trace and microprobe techniques (Print), ISSN 0733-4680, E-ISSN 1532-2270, Vol. 14, no 3, 615-631 p.Article in journal (Refereed) Published
A new freeze-substitution method using tetrahydrofuran (THF) as organic solvent was tested in the preparation of pancreas for determination of elemental content and distribution. Particle induced X-rayemission (PIXE) allowed analysis at the cellular level and X-ray microanalysis (EPMA) at the subcellular level. Using PIXE allowed detection of Zn, Ca and Fe, not detectable in the EPMA spectra. The ratios of Cl and K to P and to S obtained from analysis using both PIXE and EPMA agreed well. A good preservation of the in vivo ionic content was manifested in high K to Na and K to Cl ratios.
Place, publisher, year, edition, pages
1996. Vol. 14, no 3, 615-631 p.
IdentifiersURN: urn:nbn:se:uu:diva-35321ISI: A1996VA18600008OAI: oai:DiVA.org:uu-35321DiVA: diva2:63221