Direct measurement of elemental distributions in insulin-producing cells using nuclear microscopy
1996 (English)In: Cellular and Molecular Biology, ISSN 0145-5680, Vol. 42, no 1, 49-57 p.Article in journal (Refereed) Published
The nuclear microprobe was used to study elemental distributions in cultured insulin-producing cellsand in pancreatic tissue. To see whether insulin producing cells are polarized, the cells were stimulatedin the presence of Sr as a Ca analogue. The cells were stimulated to secrete insulin and Sr was detected in cells after momentary stimulation. We did not detect a polarized distribution of Sr in the RINm5F-cells. The distributions of Ca and Zn were affected when beta-cells (ob/ob) from a primary culture flattened out onto a substrate. In uprounded beta-cells (ob/ob) the distribution of Ca and Zn was polarized. Pancreatic tissue (ob/ob) was prepared for elemental analysis using freeze-substitution intetrahydrofurane (THF) followed by embedding in Araldite. The resulting samples can be sectioned at room temperature, easing physiological studies.
Place, publisher, year, edition, pages
1996. Vol. 42, no 1, 49-57 p.
IdentifiersURN: urn:nbn:se:uu:diva-35322ISI: A1996UA90900006OAI: oai:DiVA.org:uu-35322DiVA: diva2:63222