Ion dynamics in cells-preparation for studies of intracellular processes
1995 (English)In: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, ISSN 0168-583X, Vol. 104, no 1-4, 324-327 p.Article in journal (Refereed) Published
A proton beam of about 1 mu m allows the study of inner structures of cells. These studies demand sophisticated preparation methods, not to destroy the morphology or the elemental distribution. Analysing a well-preserved cell may lead to important knowledge about basic regulatory processes at the cellular level. Freezing followed by removal of water by drying or by substitution with an organic solvent will be exemplified. Insulin-producing cells were studied to reach a further understanding of the signal transduction between stimulation to secrete insulin and the secretion.
Place, publisher, year, edition, pages
1995. Vol. 104, no 1-4, 324-327 p.
IdentifiersURN: urn:nbn:se:uu:diva-35340DOI: 10.1016/0168-583X(95)00422-XISI: A1995RZ51300055OAI: oai:DiVA.org:uu-35340DiVA: diva2:63240
4th International Conference on Nuclear Microprobe Technology and Applications; SHANGHAI INT HOUSE EDUC, SHANGHAI, PEOPLES R CHINA; OCT 10-14, 1994