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Site directed mutagenesis on a recombinant lectin domain with the aim for increased solubility
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Biology Education Centre.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

A series of mutations were performed on a recombinant fucose binding lectin domain from the fungi Aleuria aurantia in order to break oligomer formation. The mutated variants showed lowered solubility than wild type, indicating that the oligomer formation was inhibited and is important to solubilize the protein

Place, publisher, year, edition, pages
2013. , 32 p.
Series
UPTEC X, 13 021
National Category
Engineering and Technology Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-202795OAI: oai:DiVA.org:uu-202795DiVA: diva2:633653
External cooperation
Linköpings universitet
Educational program
Molecular Biotechnology Engineering Programme
Supervisors
Examiners
Available from: 2013-06-27 Created: 2013-06-27 Last updated: 2013-07-04Bibliographically approved

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CiteExportLink to record
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