uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Expression of weight and aggression regulating genes in Drosophila melanogaster after exposure to the behavioural pheromone 11-cis-vaccenyl acetate
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
2013 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Obesity is a growing public health issue with few effective treatments. Many of the human genes involved in weight regulation have homologues in other species. In Drosophila melanogaster, 11-cis-vaccenyl acetate (cVA) is a pheromone shown to affect both aggression and feeding behaviour. The aim of this study was to investigate the effect of cVA exposure on gene expression in wild type flies and to confirm crosses with genetic knockouts in the cVA pathway. The genes studied in the wild type flies were Akh (homologue of glucagon), Dilp2,-3 and -5 (homologues of insulin), Dsk (homologue of cholecystokinin), sNPF (homologue of neuropeptide Y) and TβH. The knockout genes studied were Or65a, Or67d and TβH. RNA was extracted from whole heads, cDNA synthesis was performed and the cDNA was then used in SYBR Green RT-qPCR. The knockout genes were not confirmed, due to methodological problems. The expression of Dilp3, Dilp5 and Dsk were significantly lower in the experimental flies, the expression of the other genes where not affected. The results indicate that exposure to cVA affects the expression of some of the neuropeptides involved in weight regulation. Due to the methodological problems experienced, a recommendation is to confirm the data using different reagents.

Place, publisher, year, edition, pages
2013. , 20 p.
Keyword [en]
feeding behaviour, neuropeptides, weight regulation, aggressive behaviour, human homologues
National Category
Biomedical Laboratory Science/Technology
URN: urn:nbn:se:uu:diva-203058OAI: oai:DiVA.org:uu-203058DiVA: diva2:634945
Educational program
Biomedicinska analytikerprogrammet
Available from: 2013-08-12 Created: 2013-07-02 Last updated: 2013-08-12Bibliographically approved

Open Access in DiVA

fulltext(497 kB)264 downloads
File information
File name FULLTEXT01.pdfFile size 497 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Search in DiVA

By author/editor
Edberg, Carina
By organisation
Department of Women's and Children's Health
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 264 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 519 hits
ReferencesLink to record
Permanent link

Direct link