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Drug loading and release of Tobramycin from Hydroxyapatite coated fixation pins
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Christian-Albrechts Universitet Kiel.
Sandvik Coromant.
Sandvik Coromant.
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2013 (English)In: Journal of materials science. Materials in medicine, ISSN 0957-4530, E-ISSN 1573-4838, Vol. 24, no 9, 2265-2274 p.Article in journal (Refereed) Published
Abstract [en]

This paper evaluates the loading and release properties of Tobramycin incorporated by adsorptive loading from a solution into plasma sprayed and biomimetically coated Hydroxyapatite (HA) fixation pins. The aim of this study is to contribute towards designing a functional implant surface offering local release of the antibiotic agent to prevent post-surgical infections. Cathodic arc deposition is used to coat stainless steel fixation pins with a bioactive, anatase phase dominated, TiO2 coating onto which a HA layer is grown biomimetically. The loading and release properties are evaluated by studying the subsequent release of Tobramycin using high performance liquid chromatography and correlated to the differences in HA coating microstructure and the physical conditions under loading. The results from these studies show that a dual loading strategy consisting of a solution temperature of 90 °C and a pressure of 6 bar during a loading time of 5 min release a sufficient amount of Tobramycin to guarantee the inhibition of Staphylococcus aureus up to 2 days for plasma sprayed HA coatings and for 8 days for biomimetic coatings. The present study emphasizes the advantages of the nanoporous structure of biomimetically deposited HA over the more dense structure of plasma sprayed HA coatings in terms of antibiotic incorporation and subsequent sustained release and provides a valuable outline for the design of implant surfaces aiming for a fast-loading and controlled, local drug administration.

Place, publisher, year, edition, pages
2013. Vol. 24, no 9, 2265-2274 p.
National Category
Medical Materials
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials
URN: urn:nbn:se:uu:diva-203448DOI: 10.1007/s10856-013-4979-1ISI: 000323289600018OAI: oai:DiVA.org:uu-203448DiVA: diva2:636571
Available from: 2013-07-10 Created: 2013-07-10 Last updated: 2016-04-21Bibliographically approved
In thesis
1. Bioactive Surgical Implant Coatings with Optional Antibacterial Function
Open this publication in new window or tab >>Bioactive Surgical Implant Coatings with Optional Antibacterial Function
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Device associated infections are a growing problem in the field of orthopaedics and dentistry. Bacteria adhering to implant surfaces and subsequent biofilm formation are challenging to treat with systemic administered antibiotics. Functionalization of implant surfaces with therapeutic coatings that are capable of inhibiting bacterial adhesion are therefore considered as a straight forward strategy to treat and prevent implant related infections.

In this thesis, the use of crystalline, arc deposited TiO2 and biomimetic hydroxyapatite (HA) coatings were evaluated with respect to their potential as antibacterial surface modifications for bone-anchored implants.

UV light induced photocatalysis of anatase dominated TiO2 coated surfaces was shown to provide a bactericidal effect against S. epidermidis under clinically relevant illumination times and doses.

Major parts of the drug release work carried out was based on biomimetic HA (HA-B) coated fixation pins. The analysis of the coating characteristics revealed that the nanoporous structure of HA-B coatings in addition to the chemical composition and surface charge are essential parameters that influence the drug carrier performance. Loading by adsorption was demonstrated to be a feasible approach to quickly incorporate antibiotics. The controlled release of antibiotics was shown to facilitate bactericidal effects against S. aureus over application-relevant time periods, even when exposed to biomechanical forces during insertion into bone model materials. Antibiotic incorporation during coating growth was shown to promote somewhat longer drug release time periods than those obtained using adsorption loading.

In summary, functionalization of implant surfaces with bioactive and biocompatible coatings is a promising concept to impact the clinical success for bone-anchored applications. The additional feature of optional, on-demand antibacterial properties of these coatings through either on-site drug release or photocatalytic antibacterial treatment is advantageous for the prevention and effective treatment of devices-associated infections. Both strategies provide an immediate response to the implant contamination by bacteria and are believed to contribute towards minimizing the origin of post-surgical infections, while at the same time improving the interfacial stability between implant and bone.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2013. 60 p.
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1091
Hydroxyapatite, titanium dioxide, photocatalysis, antibacterial effect, antibiotic release, biomimetic coating, co-precipitation, tobramycin
National Category
Medical Materials Materials Engineering
urn:nbn:se:uu:diva-209283 (URN)978-91-554-8782-9 (ISBN)
Public defence
2013-12-05, Room Å2001, Ångströmlaboratoriet, Lägerhyddsvägen 1, Uppsala, 13:00 (English)
Available from: 2013-11-13 Created: 2013-10-16 Last updated: 2014-01-23

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Lilja, MirjamBrohede, UlrikaStrømme, Maria
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