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Evaluation of VitriBlast™ for vitrification of immature oocytes
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Women's and Children's Health.
2013 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Cryopreservation of gametes and embryos is crucial in fertility treatment and fertility preservation. Preservation of oocytes is a more complicated process than preservation of sperm and embryos. According to recent studies, a new preservation technique called vitrification is found to have higher rates of oocytes-survival after warming

   The aim of this study was to evaluate VitriBlast™ Kit, for vitrification of oocytes. Vitrification is an ultra-rapid freezing method in the presence with of high concentrations of cryoprotectants which avoids intracellular ice-crystal formation during the freezing and warming process.

    In this study, a total number of 117 immature oocytes were used and 62 of these oocytes were vitrified with VitroBlast™ Kit. During this process two different vitrification devices were used, VitroLoop™ and Cryopette®. After warming, the average survival rate for vitrified oocytes was found to be 61% for VitroLoop™ and 15% for Cryopette® (p<0.001). The remaining 55 oocytes were used as a non-frozen control group and the same incubation method as for vitrified oocytes was used. The survival rate for the control groups was higher than for the vitrified groups (93% versus 35%, p<0.001). The results of this study indicate that VitriBlast™ Kit is not suitable for vitrification of oocytes.

Place, publisher, year, edition, pages
2013. , 21 p.
Keyword [en]
oocytes, fertility preservation, vitrification, VitroLoop, Cryopette
National Category
Biomedical Laboratory Science/Technology
URN: urn:nbn:se:uu:diva-203471OAI: oai:DiVA.org:uu-203471DiVA: diva2:636737
Educational program
Biomedicinska analytikerprogrammet
Available from: 2013-08-12 Created: 2013-07-11 Last updated: 2013-08-12Bibliographically approved

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