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Nanoscale Size Control of Protein Aggregates
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials. Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Karolinska Institutet.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Applied Materials Sciences.
Karolinska Institutet.
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2013 (English)In: Small, ISSN 1613-6810, E-ISSN 1613-6829, Vol. 9, no 19, 3320-3326 p.Article in journal (Refereed) Published
Abstract [en]

Herein, a novel method to synthesize soluble, sub-micrometer sized protein aggregates is demonstrated by mixing native and denatured proteins without using bacteria and contaminating proteins. Ovalbumin (OVA) is employed as a model protein. The average size of the formed aggregates can be controlled by adjusting the fraction of denatured protein in the sample and it is possible to make unimodal size distributions of protein aggregates. OVA aggregates with a size of ∼95 nm are found to be more immunogenic compared to native OVA in a murine splenocyte proliferation assay. These results suggest that the novel method of engineering size specific sub-micrometer sized aggregates may constitute a potential route to increasing the efficacy of protein vaccines. The protein aggregates may also be promising for use in other applications including the surface functionalization of biomaterials and as industrial catalysis materials.

Place, publisher, year, edition, pages
John Wiley & Sons, 2013. Vol. 9, no 19, 3320-3326 p.
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Materials Science
Identifiers
URN: urn:nbn:se:uu:diva-204000DOI: 10.1002/smll.201300149ISI: 000327899900020PubMedID: 23606559OAI: oai:DiVA.org:uu-204000DiVA: diva2:637618
Available from: 2013-07-19 Created: 2013-07-19 Last updated: 2017-12-06Bibliographically approved

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Pedersen, ChristianEngqvist, HåkanStrømme, Maria

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