uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Comparisons of Immunoassay and Mass Spectrometry Measurements of Serum Estradiol Levels and Their Influence on Clinical Association Studies in Men
Show others and affiliations
2013 (English)In: Journal of Clinical Endocrinology and Metabolism, ISSN 0021-972X, E-ISSN 1945-7197, Vol. 98, no 6, E1097-E1102 p.Article in journal (Refereed) Published
Abstract [en]

Context: Immunoassay-based techniques, routinely used to measure serum estradiol (E2), are known to have reduced specificity, especially at lower concentrations, when compared with the gold standard technique of mass spectrometry (MS). Different measurement techniques may be responsible for the conflicting results of associations between serum E2 and clinical phenotypes in men. Objective: Our objective was to compare immunoassay and MS measurements of E2 levels in men and evaluate associations with clinical phenotypes. Design and Setting: Middle-aged and older male subjects participating in the population-based Osteoporotic Fractures in Men (MrOS) Sweden study (n = 2599), MrOS US (n = 688), and the European Male Aging Study (n = 2908) were included. Main Outcome Measures: Immunoassay and MS measurements of serum E2 were compared and related to bone mineral density (BMD; measured by dual energy x-ray absorptiometry) and anklebrachial index. Results: Within each cohort, serum E2 levels obtained by immunoassay and MS correlated moderately (Spearman rank correlation coefficient r(S) 0.53-0.76). Serum C-reactive protein (CRP) levels associated significantly (albeit to a low extent, r(S) = 0.29) with immunoassay E2 but not with MS E2 levels. Similar associations of immunoassay E2 and MS E2 were seen with lumbar spine and total hip BMD, independent of serum CRP. However, immunoassay E2, but not MS E2, associated inversely with ankle-brachial index, and this correlation was lost after adjustment for CRP. Conclusions: Our findings suggest interference in the immunoassay E2 analyses, possibly by CRP or a CRP-associated factor. Although associations with BMD remain unaffected, this might imply for a reevaluation of previous association studies between immunoassay E2 levels and inflammation-related outcomes.

Place, publisher, year, edition, pages
2013. Vol. 98, no 6, E1097-E1102 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-204124DOI: 10.1210/jc.2012-3861ISI: 000319736500010OAI: oai:DiVA.org:uu-204124DiVA: diva2:637765
Available from: 2013-07-22 Created: 2013-07-22 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(317 kB)175 downloads
File information
File name FULLTEXT01.pdfFile size 317 kBChecksum SHA-512
64cf122acaae82ccdecd1bf643df2df2619d989cd68de6a72d8389aebeee5405c8ddc62cd3c5f22453ea9b4c646cdb0a7a2f98b7191e044bb6a460b43ad99ab6
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Ljunggren, Östen

Search in DiVA

By author/editor
Ljunggren, Östen
By organisation
Metabolic Bone Diseases
In the same journal
Journal of Clinical Endocrinology and Metabolism
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 175 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 400 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf